通过 Shewanella oneidensis MR-1 细胞进行多基因细胞介导的反氧传导
Shuai Xu1, Alexandre Barrozo2, Leonard M Tender3
1Department of Physics and Astronomy , University of Southern California , Los Angeles , California 90089 , United States.
Journal of the American Chemical Society
|July 31, 2018
概括
Shewanella oneidensis MR-1 细胞使用多基因细胞染色体进行细胞外电子转移 (EET). 电化学测量显示了通过Mtr通路的氧化还原导电,这对于微生物电子传输至关重要.
科学领域:
- 微生物学
- 生物物理
- 电化学
背景情况:
- 多细胞染色体促进微生物中的细胞外电子转移 (EET).
- 这些蛋白质对于微生物的新陈代谢和与固体表面的相互作用至关重要.
- 它们也与细胞内和细胞间的长距离电子传输有关.
研究的目的:
- 研究Shewanella oneidensisMR-1细胞中的电子传输机制.
- 将电化学测量与Mtr通路中的多细胞染色体的功能联系起来.
- 确定这些微生物系统中电子传输的热激活能量.
主要方法:
- 在 Shewanella oneidensis MR-1 细胞上进行了电化学隔离测量.
- 电极之间连接了电池.
- 分析了源流对门电流的依赖性.
- 用电子跳跃计算来建模通过Mtr细胞染色体的传输.
主要成果:
- 在S. oneidensis MR-1细胞中,电化学门证明了氧化还原导电机制.
- 这种导电归因于Mtr通路的多血细胞体.
- 测量的热激活能量 (0.29 ± 0.03 eV) 与通过Mtr外膜细胞染色体的电子跳跃计算一致.
结论:
- 在S. oneidensis MR-1中的多基因细胞染色体介导基于氧化还原的电子导电.
- 在这种微生物细胞外电子转移中,Mtr路径至关重要.
- 这些发现为微生物系统的微米电子运输提供了洞察力.
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