相关实验视频
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Phase Transitions and Effect of Intermolecular Forces
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通过染色体融合的型工程导致酵母的繁殖隔离
Jingchuan Luo1,2, Xiaoji Sun1, Brendan P Cormack2
1Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.
Nature
|August 3, 2018
概括
发芽酵母耐受显著的染色体减少,两种染色体菌株显示最小的缺陷. 染色体融合可以导致生殖隔离,证明基因组的强度.
科学领域:
- 遗传学
- 分子生物学
- 进化生物学
背景情况:
- 由于基因组历史事件如融合和重复,物种表现出不同的染色体数量,即使基因组大小相似.
- 人类的染色体数量与其他猿类不同,这是由于融合事件,这引发了关于对染色体变化的耐受性的问题.
研究的目的:
- 研究酵母物种对染色体数量显著变化的耐受性.
- 探索染色体减少对酵母生长,转录学和生殖隔离的影响.
主要方法:
- 使用CRISPR-Cas9基因组编辑来融合Saccharomyces cerevisiae中的染色体.
- 产生了一系列染色体数量逐渐减少的酵母菌株 (从16个减少到2个).
- 评估了不同染色体数量的菌株之间的交叉体变化,生长缺陷和子活力.
主要成果:
- 一个双染色体酵母菌株 (约. 每个6个) 显示了适度的转录基因变化和正常生长.
- 随着染色体数量降至16个以下,子活力显著下降,十二个染色体的活力不到10%.
- 具有八个,四个或两个染色体的菌株之间的同型交叉显示出出色的分泌和生存能力,这表明八个融合事件后的繁殖隔离.
结论:
- 发芽酵母对染色体数量减少具有显著的耐受性.
- 染色体融合事件足以建立酵母菌株之间的繁殖隔离.
- 这项研究为了解基因组强度和染色体数量的进化灵活性提供了一个模型.
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