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Updated: Feb 7, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
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通过蛋白质半合成揭示了Akt激酶激活机制

Nam Chu1, Antonieta L Salguero1, Albert Z Liu2

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Cell
|August 7, 2018
PubMed
概括

通过酸化激活阿克特蛋白涉及不同的机制. 在Ser473的酸化通过分子内相互作用缓解自身抑制,而在Ser477/Thr479的双酸化使用不同的途径来调节Akt活性和PIP3结合.

关键词:
射线晶体结构双基质类似物表达的蛋白质结合基因酶质谱学类化照片交叉链接

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