在LINE的异构RNP组件控制谱系特异性RNA处理
Jan Attig1, Federico Agostini2, Clare Gooding3
1The Francis Crick Institute, Midland Road 1, Kings Cross, London NW1 1AT, UK; Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK.
Cell
|August 7, 2018
概括
长间隔的核元素 (LINE) 招募RNA结合蛋白 (RBPs) 来抑制哺乳动物内核中的RNA处理. 这种调节塑造了新的转录和组织特异性外基的演变.
科学领域:
- 基因组学
- 分子生物学
- 进化生物学
背景情况:
- 哺乳动物的内子很长,在RNA处理过程中对准确的外子识别构成挑战.
- 长间隔的核元素 (LINE) 是内部的重复序列,可以影响基因调节.
研究的目的:
- 研究LINE在吸引RNA结合蛋白 (RBPs) 到哺乳动物内核中的作用.
- 了解LINE-RBP相互作用如何影响RNA处理,并对转录的进化作出贡献.
主要方法:
- 在LINE中分析RBP结合点.
- 研究像MATR3和PTBP1这样的RBP对拼接和3'终端加工的影响.
- 对年轻与老LINE的RBP结合偏好进行比较,并将它们与外显子的距离进行比较.
主要成果:
- 许多RBPs,包括MATR3和PTBP1,可以抑制RNA处理.
- 压制性RBP优先结合进化年轻的LINE,隔离它们和周围的区域.
- 较古老的LINE位于外子附近,与增强RNA处理的RBP相关,并作为组织特异性外子的来源.
结论:
- 作为压制性RBP组装的枢纽,LINE会影响RNA处理.
- 在哺乳动物中,LINE有助于新型,血统特定的转录的演变.
- LINEs及其相关RBPs的进化动态推动了转录多样化.
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