相关实验视频

Updated: Feb 6, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

作为单分子光成像的自愈染料的Tris-N-Nitrilotriacetic酸

Viktorija Glembockyte¹, Ralph Wieneke², Karl Gatterdam²

¹Department of Chemistry and Quebec Centre for Applied Materials (QCAM) , McGill University , 801 Sherbrooke Street W. , H3A 0B8 Montreal , Quebec , Canada.

Journal of the American Chemical Society

|August 8, 2018

概括

研究人员开发了自愈的trisNTA-Alexa647光体,可显著提高单分子光成像 (SMF) 的光稳定性. 这些新型染料提供了更好的光子输出和存活时间, 非常适合先进的成像技术.

科学领域:

生物物理
化学生物学
显微镜

背景情况:

光稳定性是单分子光 (SMF) 和超分辨率成像的一个关键限制.
通过将光体与光稳定剂 (如三重兴奋状态火器) 合而产生的自愈染料提供了一种提高光稳定性的策略.

研究的目的:

研究trisNTA-Alexa647光体的自愈特性和光稳定性.
评估连接器长度和刚度对这些新型光体在SMF应用中的光物理性能的影响.

主要方法:

具有不同链接特性的Alexa647标记的trisNTA结构的合成和表征.
对光子输出,生存时间和光子计数率的评估.
稳定状态和时间分辨率的光谱研究以阐明火机制.

主要成果:

通过使用短型四烯连接器获得了25倍的最大光子输出增强,其性能优于基于溶液的光稳定.
自愈机制涉及动态激发的三重状态火由Ni(II).
最佳的光物理性能取决于影响动态和静态火过程的连接器特性的合理平衡.

结论:

对于SMF来说,TrisNTA-Alexa647光体具有卓越的光稳定性和自我修复能力.
这些光体使特定的生物分子标记和延长成像持续时间.
这些发现突显了trisNTA光体在先进的单分子成像技术中的潜力.

更多相关视频

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

相关概念视频

Amino acids

Amino acids

Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible for...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

Polyprotic Acids

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:

Molecules and Compounds

Molecules and Compounds

Atoms and Molecules

Lewis Acids and Bases

Lewis Acids and Bases

In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...