结合聚合物作为用于MALDI质谱和成像的新类双模矩阵
Kilian Horatz1,2, Marco Giampà3, Yevhen Karpov1
1Institute of Macromolecular Chemistry , Leibniz-Institut für Polymerforschung Dresden e.V. , Hohe Straße 6 , 01069 Dresden , Germany.
Journal of the American Chemical Society
|August 9, 2018
概括
作为矩阵辅助激光脱离/离子化质谱 (MALDI MS) 的矩阵,研究了五种合聚合物. 这些新型矩阵表现出双模电离和用于低分子量化合物分析的卓越性能,包括MALDI MS成像.
科学领域:
- 分析化学
- 材料科学
- 生物化学
背景情况:
- 在各种研究领域中,对低分子量化合物 (LMWC) 的分析具有至关重要的作用.
- 开发具有良好的光学特性,高电离效率和LMWC范围内的MALDI静音的新MALDI矩阵对于推进代谢途径调查至关重要.
研究的目的:
- 研究五种结合聚合物作为MALDI MS和MALDI MS成像的新型矩阵.
- 评估它们的电离效率,MALDI静音以及适用于LMWC分析和生物样本成像的性能.
主要方法:
- 五种合聚合物的合成和表征:PNDI(T2),P3DDT,PTQ1,PII(T2) 和P9OFl.
- 对单个分析物和复杂的生物样品进行MALDI MS正负模式的聚合物矩阵的评估.
- 确定标准分析物的检测极限 (reserpine,胆酸).
- 在鼠脑组织的MALDIMS成像中应用P3DDT.
主要成果:
- 所有五种聚合物都作为双模矩阵运作,在正负两种模式下具有良好的分析物离子化.
- 在LMWC范围内,PNDI{T2},P3DDT,PTQ1和PII{T2}表现为MALDI静音.
- 聚合物矩阵的性能与2,5-DHB和9AA等已知矩阵相美.
- 达到了较低的检测极限 (reserpine< 164 pmol,可乐酸< 245 pmol).
- P3DDT成功启用了MALDI MS对大鼠大脑组织形态的成像.
结论:
- 结合聚合物代表了MALDI MS和MALDI MS成像的有希望,高效和多功能矩阵.
- 这些新型矩阵促进了LMWC的分析,并为改善代谢途径研究提供了潜力.
- 这些聚合物的双模式能力和MALDI静音扩大了它们在各种分析应用中的实用性.
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