将酵母新陈代谢从酒精发酵转变为脂质生成
Tao Yu1, Yongjin J Zhou1, Mingtao Huang1
1Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, 41296 Gothenburg, Sweden.
在Saccharomyces cerevisiae中的代谢工程将代谢从乙醇发酵转移到自由脂肪酸生产. 这种重新编程实现了细胞外自由脂肪酸的高产量,证明了新的生物合成途径.
科学领域:
- 生物技术
- 代谢工程
- 合成生物学
背景情况:
- 微生物的新陈代谢对最佳生长进行了严格的调节,这对高价值产品的合成构成了挑战.
- 传统的酵母新陈代谢有利于乙醇发酵,限制了其他化合物的产生,如脂质.
研究的目的:
- 重新编程Saccharomyces大脑菌的新陈代谢以提高自由脂肪酸的产生.
- 为了证明在酵母中用脂生成代替乙醇发酵的可行性.
主要方法:
- 代谢工程策略包括改变亚细胞运输和微调辅助因子供应.
- 酸盐脱碳酶的删除和适应性实验室演变.
- 过程设计以减少碳流向生物质.
主要成果:
- 获得了33.4g/L的细胞外自由脂肪酸.
- 成功地用纯脂生成代谢取代了乙醇发酵.
- 在进化的菌株中确定了重要的酸盐激酶突变,使其能够产生脂质.
结论:
- 代谢工程可以成功地将酵母碳流转向脂质生成.
- 适应性实验室进化是克服新陈代谢调节障碍的一个关键工具.
- 这项工作为基于酵母的自由脂肪酸生产奠定了基础.
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