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昆虫嗅觉受体Orco的冷EM结构
Joel A Butterwick1, Josefina Del Mármol1, Kelly H Kim2
1Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY, USA.
Nature
|August 17, 2018
概括
昆虫的气味受体 (Orco) 使用一种新的通道结构来检测各种环境化学物质. 这种对Orco同类体的结构洞察力有助于了解嗅觉系统的演变和气味调节.
科学领域:
- 生物化学
- 结构生物学
- 神经科学
背景情况:
- 昆虫依靠嗅觉受体 (ORs) 来检测环境化学物质,包括一个保守的共受体 (Orco) 和一个可变的嗅觉受体.
- 子共受体对嗅觉信号传导至关重要,也是杀虫剂的目标.
研究的目的:
- 为了确定一个Orco同质的高分辨率结构.
- 提供关于Orco受体家族及其在嗅觉歧视中的作用的结构性见解.
主要方法:
- 使用单颗粒冷电子显微镜 (cryo-EM) 来确定Orco同质体的结构.
- 该结构以3.5 Å分辨率确定.
主要成果:
- 甲同体呈现出一种新的结构,四个子单元形成一个分支成侧导管的中央孔隙.
- 甲虫四重体在膜内具有有限的子单元相互作用,由细胞质域稳定.
- 在OR中,序列保存集中在孔隙和域中,解释了结构灵活性.
结论:
- Orco结构显示出一个独特的道架构,可以容纳OR家族内的序列多样性.
- 这种结构理解有助于研究气味调节机制和昆虫嗅觉的演变.
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