使用醇透试验进行细胞透分析
Leila Peraro1, Kirsten L Deprey1, Matthew K Moser1
1Department of Chemistry , Tufts University , Medford , Massachusetts 02155 , United States.
Journal of the American Chemical Society
|August 18, 2018
概括
一种新的测定方法,即醇透测定 (CAPA),可用于高通量测量生物治疗细胞透率. 这种方法有助于优化药物输送以提高细胞和核透率.
科学领域:
- 生物化学
- 分子生物学
- 药物发现
背景情况:
- 生物治疗药物具有治疗潜力,但由于细胞透性差,在达到细胞内目标方面存在局限性.
- 开发高通量测试对于优化生物治疗细胞透和疗效至关重要.
研究的目的:
- 开发和验证一种定量,高吞吐量和分区特定的测定方法来测量生物治疗细胞透率.
- 研究影响细胞透的因素,并探索的结构透关系.
主要方法:
- 开发一种基于HaloTag的检测方法,称为基透检测 (CAPA).
- 使用CAPA在各种条件下 (度,血清,温度,时间) 量化描述细胞透.
- 使用HaloTag局部化到细胞核的细胞系评估核透.
主要成果:
- CAPA成功量化了细胞透率及其对实验条件的依赖.
- 研究了聚合和细胞透融合的结构透关系.
- 证明了核透的定量测量,扩大了测试的实用性.
结论:
- 醇透测试 (CAPA) 是衡量和优化生物治疗细胞透的多功能和有效工具.
- CAPA有助于研究影响透和结构活动关系的因素.
- 这种测试对促进细胞透生物治疗的发展具有重要价值.
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