包装 pDNA 通过聚合物 ABC 微粒同时实现合体稳定性和结构控制
Yaming Jiang1, Timothy P Lodge1,2, Theresa M Reineke2
1Department of Chemical Engineering & Materials Science , University of Minnesota , 421 Washington Avenue SE , Minneapolis , Minnesota 55455 , United States.
Journal of the American Chemical Society
|August 24, 2018
概括
使用带电纳米颗粒的DNA压缩是治疗的关键. 研究人员发现,将聚乙烯糖醇 (PEG) 冠状体添加到聚合物细胞中,显著改善了DNA复合体的稳定性和受控的细胞结构,产生了仿生色素样形式.
科学领域:
- 材料科学
- 生物技术
- 聚合物化学
背景情况:
- 通过负荷相反的纳米粒子进行DNA压缩对于开发新疗法至关重要.
- 对各种材料的应用来说,控制合电解质复合物的组成和结构是必不可少的.
研究的目的:
- 调查等离子体DNA与聚合物离子体 (AB和ABC类型) 的复合.
- 探索外非离子冠状体 (聚乙烯糖醇 - PEG) 的长度如何影响微粒体的体稳定性,大小,组成和结构.
- 了解受控DNA凝聚的细胞架构和细胞复合特性之间的关系.
主要方法:
- 两性块聚合物的自我组装成具有不同PEG冠长 (0-10kDa) 的球状.
- 使用度定位评估体稳定性.
- 使用动态和静态光散射, ζ-电位测量和低温传导电子显微镜 (cryo-TEM) 进行微粒复合体的表征.
主要成果:
- 通过添加PEG冠状体,即使在2kDa时,DNA-小细胞复合体 (小细胞复合体) 的体稳定性也得到了显著改善.
- 微粒体的大小,组成和结构与PEG块的分子量有很强的相关性.
- 微粒复合体采用类似于染色体的仿生"串珠"形态,随着PEG长度的增加,每个复合体的微粒数量减少.
结论:
- 多离子细胞有效地将DNA凝结成仿生结构.
- 调整PEG冠状长度提供了一种简单而强大的策略来控制微粒的特性.
- 这项研究提供了对核酸复合和菌结构对产生的复合物的机制性见解.
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