基于DNA的多元组件动态网络:对系统的组成和合作催化功能的等级自适应控制
Zhixin Zhou1, Liang Yue1, Shan Wang1
1Institute of Chemistry , The Hebrew University of Jerusalem , Jerusalem 91904 , Israel.
Journal of the American Chemical Society
|August 31, 2018
概括
研究人员使用核酸序列创建了自适应性宪法动态网络 (CDN). 触发器导致CDN重新配置,改变构成级别并启用新的催化功能.
科学领域:
- 超分子化学
- 系统化学
- 生物技术
背景情况:
- 立体动态网络 (CDN) 提供适应性和响应性分子系统.
- 控制CDN重新配置是设计复杂分子架构的关键.
- 基于核酸的系统为构建动态分子网络提供了多功能平台.
研究的目的:
- 构建和演示 [3 × 2] 和 [3 × 3] CDN 的自适应和层次重构.
- 研究触发器在稳定和重新配置CDN组件中的作用.
- 在重新配置的CDN中探索新兴的催化功能.
主要方法:
- 使用核酸序列构建CDN,形成 [3 × 2] 或 [3 × 3] 网络.
- 使用单链触发器进行特定CDN组件的混合和稳定.
- 采用Mg2+离子依赖的DNA酶,通过光进行成分水平的定量分析.
主要成果:
- 在应对特定触发器时,证明了CDN的适应性和层次性重新配置.
- 观察到非连接元件的上调和连接元件的下调.
- 展示了不同CDN的触发过渡以及CDN [3×3]中的新兴催化活动.
结论:
- 基于核酸的CDN可以使用外部触发器进行自适应和层次重新配置.
- 在CDN内部的动态转换使新的催化功能出现.
- 这项工作为设计具有可调节性质的复杂响应分子系统提供了框架.
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