通过核糖体分析发现的真核细胞中蛋白质复合物的共转化组合
Ayala Shiber1,2, Kristina Döring3,4, Ulrike Friedrich3,4
1Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. a.shiber@zmbh.uni-heidelberg.de.
Nature
|August 31, 2018
概括
酵母中蛋白质复合体的组合通常在翻译过程中发生,防止错误折叠和聚合. 这种共翻译过程在真核生物中整合了蛋白质合成,折叠和组装.
科学领域:
- 分子生物学
- 细胞生物学
- 生物化学
背景情况:
- 在拥挤的细胞环境中蛋白质折叠具有挑战性,有错误折叠和聚合的风险.
- 细胞利用分子辅助器进行转化折叠,但在转化过程中蛋白质复合体的组合在真核生物中尚不清楚.
- 细胞系统不同于 prokaryotes (例如,罕见的多基RNA),提出了关于配译组合协调的问题.
研究的目的:
- 系统地分析真核生物中异构蛋白质复合物的体内组合.
- 调查蛋白质复合组合是否与Saccharomyces cerevisiae中的翻译协调.
- 阐明共翻译组合及其调节的机制细节.
主要方法:
- 使用核糖体概况来确定酵母中的12个异构分子复合体的新生子单位的体内相互作用.
- 在接近残留的分辨率下分析了子单位相互作用.
- 研究了与核糖体相关的辅导体,如Hsp70 Ssb的作用.
主要成果:
- 在12个研究的异质寡合复合体中,有9个以共翻译方式组合.
- 三个没有表现出共翻译组合的复合体由专门的组合监护人调节.
- 同转化组合通常是单向的,其中一个子单元与其新生的伴侣接触,防止聚合.
- 亚单元关联的开始与核糖体道出口的相互作用域的暴露相关.
- 在组装之前,与核糖体相关的Hsp70Ssb暂时参与域和解离,防止过早的相互作用.
结论:
- 同转化子单元的结合是酵母中异质寡合体组合的普遍机制.
- 翻译,折叠和蛋白质复合组合是真核生物中集成的过程.
- 这种融合促进了适当的蛋白质功能和细胞平衡.
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