相关实验视频
Updated: Sep 4, 2026

12:28
Preparation of Highly Coupled Rat Heart Mitochondria
Published on: September 24, 2010
概括
修饰的细胞染色体c表现出定向电子转移. 从鲁到血的电子流是快速的,而从血到鲁的电子流是显著缓慢的,这表明蛋白质的影响.
科学领域:
- 生物化学 生物化学
- 生物物理学的生物物理.
- 蛋白质动力学 蛋白质动力学
背景情况:
- 细胞染色体c是电子运输中的关键蛋白质.
- 复合物可用于修改细胞染色体c,用于研究电子转移.
- 之前的研究确定了修饰的cytochrome c中电子转移的热力学和动力学.
研究的目的:
- 为了研究改的cytochrome c.中分子内电子转移的方向性.
- 为了确定蛋白质结构是否影响电子转移的速度和方向.
- 探测细胞染色体c内的电子转移过程中的速度限制步骤.
主要方法:
- 修改鱼细胞染色体c的[NH3) 5RuII/III]复合物在histidine的位置 33.
- 进行X射线晶体学以确定标签和血红组之间的距离.
- 脉冲放射溶解用于测量电子转移的速率常数.
- 通过改变标签的氧化还原潜力来逆转电子转移方向.
主要成果:
- 在血中,从Ru (II) 到Fe (III) 的电子转移以约53s-1的速率常数 (k) 发生.
- 标签与海姆组之间的距离被确定为12-16 Å.
- 电子从heme (II) 转移到Ru (III) 的速度是相反反应的10^5倍以上.
- 电子转移的速度与蛋白质构成变化相比较.
结论:
- 在基修饰的细胞染色体c中,分子内电子转移是定向的.
- 细胞染色体c的蛋白质结构可能在调解这种方向性方面发挥作用.
- 蛋白质的结构变化可能会影响或与电子转移事件结合在一起.
更多相关视频
14:24Percutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical Model
Published on: January 21, 2018
09:36Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia
Published on: December 22, 2023
相关概念视频
Electron Transport Chains
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
Electron Carriers
Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Electron Transport Chain: Complex III and IV
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...