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Updated: Feb 5, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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具有扩展准空间的工程CRISPR-Cas9核酶

Hiroshi Nishimasu1, Xi Shi2,3, Soh Ishiguro4,5,6

  • 1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. nisimasu@bs.s.u-tokyo.ac.jp nureki@bs.s.u-tokyo.ac.jp.

Science (New York, N.Y.)
|September 1, 2018
PubMed
概括

科学家设计了一种Cas9酶变体 (SpCas9-NG),以识别放松的NG原空间器相邻动机 (PAM). 这扩大了基因组编辑能力,通过准人类细胞中以前无法访问的基因组位置.

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