通过步骤扫描FTIR光谱检测的植物染色体-蛋白质相互作用
Janne A Ihalainen1, Emil Gustavsson2, Lea Schroeder3
1Nanoscience Center, Department of Biological and Environmental Science , University of Jyväskylä , Jyväskylä 40014 , Finland.
Journal of the American Chemical Society
|September 6, 2018
概括
植物染色蛋白
科学领域:
- 生物化学
- 分子生物学
- 光谱学
背景情况:
- 植物染色体是调节植物和微生物对光反应的关键光受体.
- 通过白色素染色体的光吸收,通过蛋白质结构变化启动信号级联.
- 植物染色体信号传导的精确分子机制尚不完全理解.
研究的目的:
- 阐明光激活过程中细菌菌体的动态结构变化.
- 了解植物染色体中信号传导的分子基础.
主要方法:
- 对Deinococcus radiodurans bacteriophytochrome应用的双色阶段扫描红外光谱学
- 标记同位素 (H2O和D2O) 来追踪键的动态.
- 分子动力学模拟来补充光谱数据.
主要成果:
- 在Lumi-R中介物中与 biliverdin D-环发生无序的键,在Meta-R中完全脱离,并在Pfr中重组.
- 在Meta-R到Pfr过渡过程中发现的保存PHY舌区的重新折叠.
- 在Lumi-R中检测到微秒内早期的蛋白质脊柱变化.
- 一个保存的盐桥在Lumi-R中断裂,与氨酸与D环C=O相互作用.
结论:
- 这项研究揭示了光激活过程中染色体周围的键的逐步破坏和重组.
- 保存的PHY舌头重新折叠与最终的信号状态过渡有关.
- 这些发现为染色体异构化与全球蛋白质结构变化之间的联系提供了分子解释,从而促进了植物染色体机制的理解.
相关概念视频
02:28Leaky Scanning
5.7K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.7K
02:00Photoreceptors and Plant Responses to Light
28.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
28.5K
03:08Rate-Determining Steps
37.3K
Relating Reaction Mechanisms
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
37.3K
02:24Regulation of Expression Occurs at Multiple Steps
26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K
01:18Steps in Outbreak Investigation
592
In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
592
01:14Steps in the Modeling Process
683
Albert Bandura's theory of observational learning identifies four critical processes: attention, retention, motor reproduction, and reinforcement or motivation.
Attention is the first necessary component for observational learning. It involves focusing on what the model is doing and saying. For example, if you decide to take a drawing class to enhance your skills, you need to pay close attention to the instructor's words and hand movements. The characteristics of the model significantly...
Attention is the first necessary component for observational learning. It involves focusing on what the model is doing and saying. For example, if you decide to take a drawing class to enhance your skills, you need to pay close attention to the instructor's words and hand movements. The characteristics of the model significantly...
683