自然CRISPR-Cas12a抑制剂的系统发现
Kyle E Watters1, Christof Fellmann1,2, Hua B Bai1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|September 8, 2018
概括
研究人员在人类细胞中发现了三种新的CRISPR-Cas12a基因组编辑抑制剂. 这一发现为控制CRISPR-Cas12a技术和发现更多微生物抗CRISPR蛋白开辟了新的途径.
科学领域:
- 分子生物学
- 基因组学
- 生物技术
背景情况:
- CRISPR-Cas12a (Cpf1) 是一种广泛用于基因组工程的多功能核酶.
- 与CRISPR- Cas9和Cas3系统不同的是,CRISPR- Cas12a的已知抑制剂以前未被发现.
- 缺乏抑制剂限制了对CRISPR-Cas12a应用的精确控制.
研究的目的:
- 在人类细胞中发现新型CRISPR- Cas12a活性抑制剂.
- 探索CRISPR自我向和反CRISPR流行之间的关系.
主要方法:
- 使用全面的生物信息和实验查方法.
- 对其阻断或减少CRISPR-Cas12a介导的基因组编辑的能力进行了测试.
- 对原生生物基因组进行了分析,以查找CRISPR自我定位和反CRISPR元素之间的联系.
主要成果:
- 确定了三种不同的抑制剂,有效地阻断或减少CRISPR-Cas12a在人体细胞中的基因组编辑.
- 发现了CRISPR自我向与细菌基因组中抗CRISPRs存在的显著相关性.
- 这表明了发现许多新抗CRISPR的潜在机制.
结论:
- 发现CRISPR-Cas12a抑制剂为精确的基因组工程提供了至关重要的工具.
- 这些发现提高了CRISPR-Cas12a在各种领域的可控性和安全性.
- 自我向和抑制剂流行之间的联系为未来的抗CRISPR发现提供了有希望的策略.
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