Jove
Visualize
联系我们

相关概念视频

Bond Energies and Bond Lengths02:49

Bond Energies and Bond Lengths

31.5K
Stable molecules exist because covalent bonds hold the atoms together. The strength of a covalent bond is measured by the energy required to break it, that is, the energy necessary to separate the bonded atoms. Separating any pair of bonded atoms requires energy — the stronger a bond, the greater the energy required to break it.
31.5K
Peptide Bonds02:43

Peptide Bonds

83.1K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
83.1K
Bonding in Metals02:32

Bonding in Metals

52.5K
Metallic bonds are formed between two metal atoms. A simplified model to describe metallic bonding has been developed by Paul Drüde called the “Electron Sea Model”. 
52.5K
Ionic Bonds00:42

Ionic Bonds

131.0K
Overview
When atoms gain or lose electrons to achieve a more stable electron configuration they form ions. Ionic bonds are electrostatic attractions between ions with opposite charges. Ionic compounds are rigid and brittle when solid and may dissociate into their constituent ions in water. Covalent compounds, by contrast, remain intact unless a chemical reaction breaks them.
Opposing Charges Hold Ions Together in Ionic Compounds
Ionic bonds are reversible electrostatic interactions between ions...
131.0K
Valence Bond Theory02:45

Valence Bond Theory

50.2K
Overview of Valence Bond Theory
50.2K
Covalent Bonds01:29

Covalent Bonds

162.8K
Overview
162.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Plasma-SELEX for Acute Myocardial Infarction Biomarker Discovery and Diagnosis.

Analytical chemistry·2026
Same author

Uncovering hidden cross-regional environmental risks: Network evidence from off-site penalties and implications for pollution transfer in China.

iScience·2026
Same author

Joint Impact of Triglyceride-glucose Index and Free Fatty Acid Levels on Cardiovascular Outcomes in Overweight or Obese Patients with Coronary Artery Disease - A Large Multicenter Prospective Study.

Biomedical and environmental sciences : BES·2026
Same author

Reprogramming Aromatic Camptothecins into TOP1 Degraders via Synergistic Hydrophobic Tagging and Supramolecular Assembly.

Journal of the American Chemical Society·2026
Same author

Author Correction: DNA nanodevices detect an acidic nanolayer on the lysosomal surface.

Nature cell biology·2026
Same author

Receptor-Tethered Cytosolic Modulators Enable Spatial Control of Cell Signaling Specificity.

ACS nano·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Feb 5, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.4K

具有pH响应性DNA键的调节性阿普坦特

Long Li1, Ying Jiang1,2, Cheng Cui2

  • 1Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute, McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , United States.

Journal of the American Chemical Society
|September 14, 2018
PubMed
概括

通过利用pH响应的i动图,结构可切换的aptams (SW-Apts) 提供了更好的细胞向. 这些体在酸性环境中特别与细胞结合,同时在生理pH下避免非细胞.

更多相关视频

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.3K
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

3.6K

相关实验视频

Last Updated: Feb 5, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.4K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.3K
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

3.6K

科学领域:

  • 生物技术
  • 分子生物学
  • 化学生物学

背景情况:

  • 由于其特定的细胞识别,aptamers是生物分析和向治疗的关键分子工具.
  • 目标细胞和非目标细胞的共享受体可能会阻碍选择性吸收酶的识别.
  • 在复杂的生物环境中开发具有增强特异性的体是必要的.

研究的目的:

  • 设计和开发基于细胞微环境pH的可重新配置的结合亲和度的结构可切换的体.
  • 设计利用pH差异对目标细胞进行特定结合的aptamers.
  • 克服不同细胞类型共享受体引起的交叉反应的挑战.

主要方法:

  • 用响应pH的i-图形结构修改的单链体的构造.
  • 使用i-motifs (富含cytosine的四重复结构) 来调节胺酶的结合亲和力.
  • 使用Förster共振能量转移 (FRET) 和循环二极化 (CD) 光谱来验证i-motif诱导的结构切换.
  • 在不同的pH条件下 (酸性与生理性) 评估aptamer结合能力.

主要成果:

  • 开发的SW-Apt在特定的酸性pH下对细胞具有很高的结合 afinity.
  • 在生理pH下没有观察到SW- Apt的显著结合,这表明pH取决于特异性.
  • 证实i-motif结构可以诱导所需的结构切换行为.
  • SW-Apt在血清中表现出高特异性和增强的稳定性,这是由于折叠的i-motif结构.

结论:

  • 这项研究提出了一种新的策略,用于化学调节胺结合能力并增强细胞的特异性.
  • 响应pH的SW-Apt有效地区分了目标细胞和非目标细胞,即使它们有共同的受体.
  • 这种方法为提高复杂生物系统中基于aptamer的诊断和治疗效果提供了有希望的解决方案.