甲酸终结的分子识别[8]uril
Zoheb Hirani1, Hailey F Taylor1, Emily F Babcock1
1Department of Chemistry , Trinity University , 1 Trinity Place , San Antonio , Texas 78212 , United States.
Journal of the American Chemical Society
|September 18, 2018
概括
合成受体库库比特[8] (Q8) 现在以高亲和力结合N终端甲酸. 这一发现扩大了库库比的应用范围,使其能够识别非芳香和蛋白质.
科学领域:
- 超分子化学
- 化学生物学
- 分子识别
背景情况:
- 黄瓜[8] (Q8) 之前对具有芳香残留的具有很高的亲和力.
- 结合通常涉及同时识别两个芳香残留物或一种芳香残留物及其邻居.
- 非芳香通常对合成受体具有较弱的结合.
研究的目的:
- 调查Q8对含有N终端甲 (Met) 的的分子识别.
- 确定Q8是否可以与高亲和度的非芳香结合.
- 确定高亲和度Q8相互作用的序列决定因素.
主要方法:
- 使用光指示器位移试验进行体库合成和定性选.
- 使用异热定位热量计 (ITC) 进行定量结合分析.
- 使用ITC,电喷离子质谱和1H NMR光谱的详细结合决定性研究.
主要成果:
- Q8对甲基终结 (MLA,MYA,MFA) 显示出亚微分子结合亲和力.
- 证实了非芳香的高亲和度结合,挑战了以前的限制.
- 确定了甲基终结的高亲和度结合的序列决定因素.
结论:
- 酸介导的的识别不需要高亲和度的芳香残留物.
- Q8可以有效地向蛋白质中的N-终端和无序循环.
- Q8显示了针对未经修改的甲基终端蛋白质的潜力.
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