通过disenide-selenoester结合化学构建具有挑战性的proline-proline结合点
Jessica Sayers1, Phillip M T Karpati1, Nicholas J Mitchell1
1School of Chemistry , The University of Sydney , Sydney , NSW 2006 , Australia.
Journal of the American Chemical Society
|September 22, 2018
概括
研究人员开发了一种新的方法来在中产生proline-proline连接,克服了以前的局限性. 这一突破使得以前认为不可能的富含蛋白质合成成为可能.
科学领域:
- 生物化学
- 有机化学
- 化学生物学
背景情况:
- 聚氨酸序列对于原核生物和真核生物的二次结构至关重要.
- 由于体阻碍和C端体残留物的反应性降低,合成素-素基因具有挑战性.
研究的目的:
- 开发一种新的化学结合策略来构建难以捉摸的-结.
- 为了使复杂的和蛋白质能够合成.
主要方法:
- 使用了prolyl selenoesters和一个超γ-selenoproline部分.
- 采用了一种脱化-脱化.
- 开发了一种单组装工艺.
主要成果:
- 首次成功合成了proline-proline结合物.
- 实现了富含proline的单组合,包括下腺调节蛋白3B和隆布里辛-1.
- 证明了新型结合化学的效率.
结论:
- 开发的方法克服了和蛋白质构造方面的重大合成挑战.
- 这种化学反应使得以前难以处理的富含proline的蛋白质目标.
- 能够合成具有结构复杂性的和蛋白质.
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