人类骨干细胞的鉴定
Charles K F Chan1, Gunsagar S Gulati1, Rahul Sinha2
1Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
Cell
|September 23, 2018
概括
研究人员从骨,iPSC和脂肪组织中分离出自我更新,多功能的人类骨干细胞 (hSSC). 这些hSSCs产生骨,软骨和肌,帮助骨修复和支持造血干细胞.
科学领域:
- 复原医学
- 发育生物学
- 干细胞生物学
背景情况:
- 人类骨干干细胞 (hSSCs) 的层次组织和调节尚未得到充分理解.
- 识别和描述hSSC对于了解骨发育和修复至关重要.
研究的目的:
- 隔离和表征自我更新的人类骨干细胞 (hSSC).
- 调查hSSC在产生各种骨血统中的潜力及其在骨损伤中的作用.
- 将人类和小鼠骨干干细胞之间的基因表达和表观遗传数据进行比较.
主要方法:
- 从胎儿和成年人骨中分离hSSCs,BMP2治疗的脂肪层 (B-HAS) 和诱导的多能干细胞 (iPSCs).
- 分离hSSC的单细胞基因表达分析.
- 评估hSSC对骨,软骨和肌的分化潜力.
- 对骨损伤的hSSC反应的体内研究.
- 对血造干细胞维护的hSSC衍生层的分析.
- 人类和小鼠骨干干细胞 (mSSC) 的基因表达和表观遗传数据的比较分析.
主要成果:
- 能够产生骨,软骨和肌但不产生脂肪的自我更新和多能hSSCs的隔离.
- 在胎儿和成人的骨头中发现了hSSCs,并且可能来自B-HAS和iPSC.
- 基因表达分析显示了来自不同来源的hSSCs之间的相似性,其中一些偏向于胎儿和iPSC衍生的细胞的软骨分化.
- 在急性骨损伤时,hSSCs表现为局部扩张.
- 在无血清条件下维持hSSC衍生的人体造血干细胞 (hHSC).
- 在人类和小鼠SSC之间的骨架发生过程中确定保存和分离的途径.
结论:
- 一个独特的自我更新的人类骨干干细胞 (hSSCs) 已被识别和描述.
- hSSCs在骨修复中发挥作用,可以支持造血干细胞,突出显示它们的治疗潜力.
- 对比分析提供了跨物种骨干细胞功能的保存和分离机制的见解.
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