关于"DNA损伤是排序错误的普遍原因,直接混变体识别"的评论
Chip Stewart1, Ignaty Leshchiner1, Julian Hess1
1Broad Institute of MIT and Harvard, Cambridge, MA 02124, USA.
概括
测序DNA损伤的文物可能会影响研究结果. 之前的研究,包括"癌症基因组图谱",已经确定并解决了这些DNA测序问题.
科学领域:
- 基因组学
- 分子生物学
- 生物信息学
背景情况:
- 在样本处理过程中的DNA损伤可以引入测序文物.
- 这些文物可能导致遗传研究的错误结论.
- 之前的研究已经发现并减轻了类似的文物.
研究的目的:
- 对陈等人提出的研究结果进行批判性评价. 关于DNA测序的文物.
- 为了纠正陈等人手稿中的错误.
- 为了突出先前对测序文物的工作.
主要方法:
- 由陈等人对手稿的审查.
- 与已建立的协议和大规模测序项目的结果进行比较.
- 对DNA损伤引起的测序错误的分析.
主要成果:
- 陈等人的手稿. 包含事实上的错误.
- 陈等人得出的结论 由于这些错误而无法支持.
- 讨论的测序文物之前已被识别和管理.
结论:
- 陈等人的研究结果. 由于方法上的错误和之前的研究被忽视而存在缺陷.
- DNA损伤诱导的测序文物问题已被认可,并已在主要的基因组学计划中得到解决.
- 研究人员应了解有关测序人工物减轻现有文献.
相关概念视频
Nucleotide Excision Repair
Overview
Overview of DNA Repair
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
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Nucleotide Excision Repair
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DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Overview of DNA Repair
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
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Nucleotide Excision Repair
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...


