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相关概念视频

What is Gene Expression?01:42

What is Gene Expression?

196.8K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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What is Gene Expression?01:36

What is Gene Expression?

11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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5.6K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
Histone Modification02:32

Histone Modification

16.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.2K

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相关实验视频

Updated: Feb 4, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

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在发育过程中调节基因表达的RNA修饰

Michaela Frye1,2, Bryan T Harada3,4, Mikaela Behm5

  • 1Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK. m.frye@gen.cam.ac.uk chuanhe@uchicago.edu.

Science (New York, N.Y.)
|September 29, 2018
PubMed
概括
此摘要是机器生成的。

调节基因表达,对发育至关重要. 像N6-甲基氨酸 (m6A) 这样的信使RNA (mRNA) 修改会影响转录稳定性和翻译,协调细胞过程.

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Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

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Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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科学领域:

  • 分子生物学
  • 遗传学
  • 生物化学

背景情况:

  • RNA修饰是基因表达的关键转录后调节者.
  • 这些修改影响了多种真核生物过程,对正常发育至关重要.
  • 传递 RNA (mRNA) 的修饰会影响 mRNA 的代谢,包括翻译和稳定性.

研究的目的:

  • 突出RNA修饰在基因表达中的调节作用.
  • 解释像N6-甲基氨酸 (m6A) 这样的特定修饰如何协调转录调节.
  • 强调RNA修饰在细胞过程和发育中的重要性.

主要方法:

  • 对RNA修饰的现有文献的审查.
  • 分析N6-甲基氨酸 (m6A) 对mRNA代谢的影响.
  • 检查与RNA结构,功能和对外部线索的反应相关的tRNA修饰.

主要成果:

  • 对于真核生物的基因表达和发育来说,RNA的修改至关重要.
  • N6-甲基氨酸 (m6A) 调节mRNA的翻译和稳定性,协调转录组.
  • 转移RNA (tRNA) 修改对RNA结构和功能至关重要,并且适应刺激的蛋白质合成.

结论:

  • 在协调基因表达程序中,RNA的修改起着基本的作用.
  • 这些修改对于维持细胞状态和促进发育过渡至关重要.
  • 正确的RNA修饰的沉积和功能对于正常的真核生物发育是必不可少的.