在c-fos基因控制中涉及常见和细胞类型特定的途径:在巨细胞中稳定诱导cAMP
Cell
|January 30, 1987
概括
血小板衍生生长因子 (PDGF) 和殖民地刺激因子1 (CSF-1) 分别在纤维细胞和巨细胞中暂时诱导c-fos. 循环AMP (cAMP) 诱导了巨细胞中稳定的c-fos转录,表明了不同的调节机制.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 基因规则 基因规则
背景情况:
- 通过PDGF和CSF-1等外部刺激,c-fos原瘤基因被迅速诱导.
- 脂分解,蛋白激酶C (PKC) 和Ca2+释放都与c-fos诱导有关.
- 卡尔莫杜林在c-fos mRNA降解中的作用是由其抑制作用所暗示的.
研究的目的:
- 研究调节纤维细胞和巨细胞中c-fos原型基因诱导的机制.
- 探索信号通路的差异性作用,包括PKC,Ca2+,calmodulin和循环核酸,在c-fos基因表达中.
主要方法:
- 纤维细胞和巨细胞的细胞培养.
- 用生长因子 (PDGF,CSF-1) 和信号调节剂 (calmodulin 抑制剂,循环核酸) 的治疗.
- 对c-fos mRNA和蛋白质水平以及基因转录率的分析.
主要成果:
- PDGF在纤维细胞中暂时诱导c-fos;CSF-1在巨细胞中暂时诱导c-fos.
- PKC和Ca2+信号通路参与了c-fos的暂时诱导.
- 卡尔莫杜林的抑制延长了PDGF的c-fos mRNA和蛋白质诱导.
- 与纤维细胞不同的是,cAMP在巨细胞中强烈诱导稳定的c-fos转录.
结论:
- 不同的信号通路调节c-fos原型瘤基因表达,以应对不同的刺激.
- 卡尔莫杜林在调节c-fos mRNA的降解方面发挥着作用.
- 巨细胞与纤维细胞相比,通过cAMP对c-fos转录的调节有差异,这表明细胞特异性机制.
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