一个ESIPT探测器用于以Aβ聚合物结合促进的氧化的比度成像
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Adam C Sedgwick1,2, Wei-Tao Dou3, Jin-Biao Jiao3
1Department of Chemistry , University of Bath , Bath BA2 7AY , U.K.
Journal of the American Chemical Society
|October 3, 2018
概括
新的光探测器检测过氧化物 (ONOO-) 和图像粉素-β (Aβ) 聚合物. 这一突破使得纤维蛋白和活性氧/物种的同时检测成为可能.
科学领域:
- 生物化学
- 化学生物学
- 材料科学
背景情况:
- 粉样蛋白-β (Aβ) 聚合与神经退行性疾病有关.
- 过氧 (ONOO-) 是一种参与氧化应激和疾病发病的活性物种.
- 开发用于同时检测Aβ和ONOO-的选择性探针具有挑战性.
研究的目的:
- 开发基于3-基黄 (3-HF) 的新型光探针,利用激发状态的分子内质子转移 (ESIPT) 来检测ONOO.
- 调查探测器在ONOO-的存在下对Aβ的不同聚合状态的成像能力.
- 探索用于同时探测Aβ和ONOO-的宿主-客组合的发展.
主要方法:
- 基于酸的光探针的合成.
- 在微粒环境中对ONOO-的探测响应的评估.
- 在ONOO-的存在下使用探针对Aβ聚合物进行成像.
- 当与Aβ聚合物结合时,3-HF-OMe探针的比度响应的表征.
主要成果:
- 开发了基于酸盐的3-HF ESIPT光探测器,具有对环境敏感的特性.
- 探测器在微粒环境中对ONOO-的比度反应.
- 在ONOO-的存在下成功成像了Aβ的不同聚合状态.
- 3-HF-OMe探测器在与Aβ聚合物结合时表现出比度反应,形成了一个新的宿主-客体组合.
结论:
- 开发的3HF ESIPT探测器可以有效地检测ONOO-.
- 这些探测器可以在ONOO-存在的背景下可视化Aβ聚合状态.
- 3-HF-OMe探测器的宿主-客组合提供了一种同时检测纤维蛋白和反应性物种的新策略.
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