Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

High-fidelity rare structural variant detection with HiFiRE3 reduced representation via restriction enzyme ends.

bioRxiv : the preprint server for biology·2026

Same author

Maturation of HIV-1 neutralizing antibodies in a germinal center conditional expression mouse model.

PLoS pathogens·2026

Same author

Diverse germline-targeting HIV Env immunogens select for distinct mutations in the same knock-in mice B cell receptors.

Nature communications·2026

Same author

Author Correction: Defective DNA damage repair leads to frequent catastrophic genomic events in murine and human tumors.

Nature communications·2026

Same author

Linear RAG scanning mediates editing of Igκ variable region repertoires.

Nature·2026

Same author

Recurrent SARS-CoV-2 Omicron broadly neutralizing humanized antibodies in different single human V<sub>H</sub>1-2-rearranging mouse models.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

相关实验视频

Updated: Jul 8, 2026

Immune Compatible Blood Transfusion and Agglutination

Immune Compatible Blood Transfusion and Agglutination

神经母细胞补充使小鼠前脑产生器官

Amelia N Chang¹, Zhuoyi Liang¹, Hai-Qiang Dai¹

¹Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics and Department of Pediatrics, Harvard Medical School, Boston, MA, USA.

|October 12, 2018

概括

神经母细胞补充 (NBC) 能够通过为捐赠干细胞创造一个空缺的利基来研究前脑发育. 这种方法快速生成复杂的小鼠模型用于大脑器官生成和功能分析.

更多相关视频

Disorders of Erythrocytes

Disorders of Erythrocytes

Rh Blood Group

Rh Blood Group

相关实验视频

Last Updated: Jul 8, 2026

Immune Compatible Blood Transfusion and Agglutination

Immune Compatible Blood Transfusion and Agglutination

Disorders of Erythrocytes

Disorders of Erythrocytes

Rh Blood Group

Rh Blood Group

科学领域:

神经科学
发育生物学
遗传学

背景情况:

产生基因改造小鼠的传统方法,如ES细胞微注射,需要广泛的繁殖来进行生殖线传播.
以前用于免疫系统和器官研究的胚胎细胞补充剂尚未适应大脑发育.
由于前脑发育的复杂性,研究大脑中的基因功能具有挑战性.

研究的目的:

为研究前脑发育和功能开发一种新的方法 - - 神经胚胎细胞补充 (NBC).
使用小鼠模型创建一种分析前脑发育所必需的基因功能系统.
建立一个快速有效的方法来生成复杂的前脑器官生成模型.

主要方法:

在宿主胚胎中使用甲状腺毒素A子单元对来自宿主的背部脑前代进行定向切除.
将捐赠胚胎干细胞 (ES) 注入前脑特异性的胚胎囊中.
使用CRISPR-Cas9生成缺少基因的ES细胞 (例如,双皮质缺乏) 用于补充研究.

主要成果:

NBC成功地创造了一个空缺的前脑,导致宿主胚胎中大脑皮质和海马体的产生.
捐赠者ES细胞填充了空缺的位置,导致形态和神经上正常的新皮质和海马.
具有双素缺乏ES细胞的NBC仿真精确地复制了常规淘汰小鼠的表型,验证了模型.

结论:

神经母细胞补充 (NBC) 是一种快速有效的技术,用于生成前脑发育和功能的复杂小鼠模型.
进行前脑器官生成和神经功能所需的基因研究.
这种胚胎细胞补充策略在研究器官生成方面具有更广泛的应用潜力.