相关实验视频

Updated: Feb 3, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

通过MYC诱导的癌症治疗来准渐进式转录延长

Kaiwei Liang¹, Edwin R Smith², Yuki Aoi¹

¹Simpson Querrey Center for Epigenetics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

|October 20, 2018

概括

研究人员发现KL-1和KL-2可以抑制超延长复合体 (SEC). 这种抑制影响转录,热冲击反应和MYC活性,显示出治疗MYC驱动的癌症的潜力.

科学领域:

分子生物学
生物化学
癌症研究

背景情况:

超延长复合体 (SEC) 通过促进RNA聚合酶II (Pol II) 释放和过程性,对有效的基因转录至关重要.
这种功能障碍与包括癌症在内的各种人类疾病有关.

研究的目的:

为了识别破坏SEC功能的小分子.
在癌症中研究SEC抑制剂的治疗潜力.

主要方法:

胺化合物KL-1和KL-2的鉴定
评估KL-1和KL-2对Pol II释放和转录延长率的影响.
评估SEC抑制对不同物种热冲击基因诱导的影响.
分析MYC和MYC依赖基因的下调.
在MYC驱动癌症的老鼠异种移植模型中测试疗效.

主要成果:

发现KL-1和KL-2是破坏AFF4-P-TEFb相互作用的化合物.
这些化合物阻碍了Pol II从休息点释放,并降低了转录延长率.
通过KL-1和KL-2抑制Drosophila和人类细胞的热冲击反应.
在哺乳动物细胞中抑制SEC可降低MYC及其下游转录位.
在MYC驱动癌症的小鼠模型中,KL- 1和KL- 2的治疗延迟了瘤的进展.

结论:

针对SEC的小分子抑制剂,如KL-1和KL-2,可以有效地破坏转录和细胞应激反应.
通过降低瘤转录程序来治疗MYC驱动的癌症,SEC抑制显示出治疗潜力.

关键词:

其他国家其他国家过程延长发起者-近端暂停超延长复合体转录延长癌症中的转录成

更多相关视频

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects TEdeff in Cancers

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects TEdeff in Cancers

Published on: September 26, 2019

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Published on: December 1, 2016

相关概念视频

Transcription Elongation Factors

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...

Transcription Elongation Factors

Transcription Elongation Factors

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Cancer Therapies

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...