双功能免疫蛋白保护细菌免受FtsZ向的ADP-Ribosylating毒素的影响
See-Yeun Ting1, Dustin E Bosch2, Sarah M Mangiameli3
1Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Cell
|October 23, 2018
概括
细菌使用ADP-ribosylating毒素进行竞争,准细胞分裂所必需的FtsZ蛋白. 一种新发现的毒素改变了FtsZ, 阻断了聚合,
科学领域:
- 微生物学
- 生物化学
- 结构生物学
背景情况:
- ADP-ribosylation是影响蛋白质功能的一个关键的翻译后修饰.
- 细菌毒素利用ADP-ribosylation来破坏真核细胞过程.
- 之前没有研究过ADP-ribosylation在细菌物种间竞争中的作用.
研究的目的:
- 研究ADP-ribosylation在细菌物种间竞争中的作用.
- 识别和描述细菌ADP-ribosylating毒素及其标.
- 阐明毒素抑制和免疫的机制.
主要方法:
- 鉴定了来自Serratia proteamaculans的ADP-ribosylating毒素.
- 毒素抑制剂复合物的结构确定.
- 生物化学测试以评估FtsZ聚合和毒素活性.
- 对毒素抑制和免疫机制的分析.
主要成果:
- 在跨物种竞争中发现的细菌ADP-ribosylating毒素.
- 证明了Serratia proteamaculans毒素修改了基本的FtsZ蛋白质,抑制了聚合和细胞分裂.
- 发现了两种不同的抑制模式:活性部位的封闭和酶的ADP- 核糖去除.
- 通过ADP-ribose去除对非同类毒素的广泛免疫力.
结论:
- 细菌军备竞赛推动了新型毒素防御系统的发展.
- 用ADP-ribosylation准FtsZ是一种细菌竞争的策略.
- 酶去除ADP- 核糖提供广泛的免疫力,保护细菌细胞分裂机制.
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