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Expanding the Toolkit for In Vivo Imaging of Axonal Transport
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通过拓素的拓逆向扩展光遗传学工具包
Jennifer Brown1, Reza Behnam2, Luke Coddington2
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, UK.
Cell
|October 23, 2018
概括
研究人员通过逆转它们的膜拓来设计素, 为神经科学创造新的工具. 这种拓工程方法产生了一种Channelrhodopsin变体,作为一种强大的抑制剂,扩大了光遗传工具包.
科学领域:
- 神经科学
- 分子生物学
- 生物物理
背景情况:
- 精确操纵神经活动对于理解大脑功能和行为至关重要.
- 光遗传学利用光敏感的微生物素,为电路解剖提供高时间精度.
- 现有的素在选择性,动力学和光谱性质上有局限性,需要新的工具.
研究的目的:
- 引入一种称为"拓工程"的新型蛋白质工程策略.
- 证明逆转膜拓可以产生具有独特功能的变体.
- 扩大用于电路神经科学研究的工具包.
主要方法:
- 开发了一种"拓工程"方法,涉及到等离子体膜内的反转.
- 创建和描述了具有改变膜拓的新型素变体.
- 测试了工程素的功能性质,包括它们的活性和动力学.
主要成果:
- 证明拓工程可以产生具有独特功能特征的opsin变体.
- 展示了一个具体的例子,其中一个Channelrhodopsin变体在逆转时从激活剂转变为快速起作用的抑制剂.
- 这种反向的素作为一个阴离子,
结论:
- 膜拓代表了对蛋白质工程的有价值和正交的维度.
- 拓工程显著扩大了光遗传工具包的多样性和实用性.
- 这种方法可能会使可用于神经科学应用的opsins数量增加一倍.
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