概括
转录因子AP-1 (激活蛋白-1) 被净化,并被证明可以激活特定的基因转录. AP-1结合部位作为瘤促进剂诱导增强剂起作用,这表明AP-1可以作为瘤促进剂诱导增强剂.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 转录因子 转录因子
背景情况:
- 增强剂结合蛋白AP-1 (激活蛋白1) 在基因调节中起作用.
- 在转录激活中AP-1的功能的精确机制及其对细胞刺激的反应需要进一步阐明.
研究的目的:
- 为了净化和表征增强剂结合蛋白AP-1.
- 研究AP-1在特定基因的转录激活中的作用,包括人类金属氨酸IIA (hMT IIA) 基因.
- 为了确定AP-1结合部位是否可以作为可诱导增强剂来响应细胞信号.
主要方法:
- 从HeLa细胞中净化AP-1,使用特定序列的DNA亲属性染色体.
- 在体外转录试验中使用野生类型和突变hMT IIA促进体.
- DNAase I保护分析以确定hMT IIA,SV40和人类原酶基因中的AP-1结合位.
- 用含有合成AP-1结合位的等离子体构造感染HeLa细胞.
主要成果:
- AP-1被净化到>95%的同质性作为一个47kd的多.
- 纯化AP-1激活了野生类型hMT IIA基因的转录,但没有缺少AP-1位点的突变促进体.
- 一个保存的TGACTCA基因被确定为hMT IIA,SV40和人类原酶基因中的AP-1结合部位.
- 合成的AP-1结合部位在转染的HeLa细胞中充当了TPA诱导增强剂.
结论:
- AP-1是一种转录因子,它与特定的增强剂元素结合.
- AP-1的活性可能由TPA (12-O-tetradecanoylphorbol-13-乙酸) 调节,这是一个已知的激活蛋白激酶C的瘤促进剂.
- AP-1结合部位调解TPA诱导基因转录,突出显示它们在细胞对外部刺激反应中的作用.
相关概念视频
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...


