在细胞基质界面上对地形景观的现场印记
Derek S Hernandez1, Eric T Ritschdorff1, Jodi L Connell1
1University Station, A5300, Department of Chemistry , The University of Texas at Austin , Austin , Texas 78712 , United States.
Journal of the American Chemical Society
|October 24, 2018
概括
研究人员开发了一种使用激光动态改变细胞培养表面的新方法. 这种技术可以在不损害细胞或基质的情况下创建,删除或逆转蛋白质水凝上的地形特征.
科学领域:
- 生物材料科学
- 细胞生物学
- 表面工程
背景情况:
- 原生环境中的附着细胞对动态的地形线索做出反应,影响分化,定向和迁移.
- 研究这些细胞反应需要体外工具,可以创建动态,任意和可逆的表面地形.
- 现有的方法往往会扰乱环境条件或损害基板表面.
研究的目的:
- 开发一种用于在细胞中强加动态地形体验的体验策略.
- 创建一种实时修改基于蛋白质的水凝表面的方法.
- 在不破坏细胞或基质完整性的情况下创建,消除和逆转表面特征.
主要方法:
- 在基于蛋白质的水凝基底中使用脉冲近红外激光聚焦.
- 通过扫描激光聚焦促进局部化学交叉链接和矩阵收缩.
- 通过动态反射面膜投射任意图案 (环,钉子,槽) 到水凝表面.
主要成果:
- 在水凝表面成功创建动态地形特征.
- 通过应用顺序扫描模式来消除或逆转创建的地形特征的能力.
- 证实激光诱导的收缩, 仅限于远离接口的3D voxels, 保持细胞活力和表面完整性.
结论:
- 开发的基于激光的策略有效地挑战了具有动态地形经验的细胞.
- 这种方法提供了一种不破坏性的方法来研究细胞拓学相互作用.
- 这种技术可以对表面地形进行精确,可逆的控制,从而促进细胞培养研究.
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