与Agouti相关的2促进了跨鱼辐射的条纹模式的融合演变
Claudius F Kratochwil1,2,3, Yipeng Liang4, Jan Gerwin4,3
1Department of Biology, University of Konstanz, Konstanz, Germany. claudius.kratochwil@uni-konstanz.de axel.meyer@uni-konstanz.de.
概括
调节性变化控制了非洲鱼中黑色条纹的演变. 减少的agrp2表达与条纹模式有关,表明其在融合进化中的作用.
科学领域:
- 进化生物学
- 遗传学
- 鱼类学
背景情况:
- 在非洲鱼的颜色图案中,表型的趋同很明显.
- 在1200多个东非裂湖物种中,
研究的目的:
- 研究非洲鱼的黑色条纹模式的融合演变的遗传基础.
- 确定这种表型的重复进化背后的分子机制.
主要方法:
- 对基因表达模式的分析.
- 关于agouti相关2 (agrp2) 的基因映射.
- 在鱼中进行CRISPR-Cas9基因编辑.
主要成果:
- 减少的agrp2表达与跨物种的条纹模式相融合.
- 在agrp2位点的cis调节突变并不普遍预测条纹.
- 基因测绘证实了agrp2位点与条纹模式的联系.
- 在非条纹鱼体中,用CRISPR-Cas9敲除了agrp2的条纹.
结论:
- 在agrp2基因中的调节变化作为条纹模式进化的分子开关.
- 独立的调节机制可能会在不同的虫辐射中驱动条纹进化.
- 一个单一的基因,agrp2,在复杂的虫颜色模式的融合进化中起着至关重要的作用.
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