PIEZOs通过神经元传感血压和巴罗受体反射
Wei-Zheng Zeng1, Kara L Marshall1, Soohong Min2
1Howard Hughes Medical Institute, Neuroscience Department, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
概括
机械激活的离子通道PIEZO1和PIEZO2对于体内血压感应机制的气体感应是必不可少的. 它们的缺失会导致小鼠的高血压和血压不稳定.
科学领域:
- 生理学
- 分子生物学
- 神经科学
背景情况:
- 呼吸反射对于急性心率和血压的调节至关重要.
- 波受体机械敏感性背后的分子机制已经难以捉摸80多年了.
研究的目的:
- 识别负责任的分子组件机械敏感性在巴罗受体神经元.
- 阐明PIEZO通道在气体感应和血压平衡中的作用.
主要方法:
- 在小鼠的感觉 (nodose 和 petrosal) 中对PIEZO1和PIEZO2进行基因切除.
- 使用药物诱导反应和主动脉压缩神经活动评估气流反射功能.
- 绝杀小鼠血压变化和可变性的表型分析.
- 具有PIEZO2阳性的感官附带体的光遗传激活.
主要成果:
- 对PIEZO1和PIEZO2的遗传删除消除了药物诱导的巴罗反射和大动脉压缩神经活动.
- 缺乏PIEZO通道的小鼠表现出不稳定的高血压和增加的血压变化.
- 对表达PIEZO2的光遗传刺激成功启动了巴罗反射反应.
结论:
- 作为主要的机械传感器,PIEZO1和PIEZO2对于气体感应是不可或缺的.
- 这些通道对于保持急性血压控制至关重要.
- 巴罗受体中PIEZO通道的功能障碍可能导致巴罗反射失效和相关的心血管疾病.
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