在单个样本中进行多重免疫试验的潜在分辨多色电化学发光
Weiliang Guo1, Hao Ding1, Chaoyue Gu2
1Institute of Analytical Chemistry, Department of Chemistry , Zhejiang University , Hangzhou 310058 , China.
Journal of the American Chemical Society
|November 2, 2018
概括
研究人员为电化学发光 (ECL) 多重免疫试验开发了新的和复合体. 这项创新使得同时检测多个生物标志物的高通量检测成为可能,通过提高灵敏度和减少样本量来推进临床诊断.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 电化学发光 (ECL) 对于临床免疫测试至关重要,但由于光的可用性而受到限制.
- 多重免疫测试需要多种,可辨别的信号报告器来同时检测生物标志物.
研究的目的:
- 为ECL应用合成和表征新型 (II) 和 (III) 复合物.
- 开发一个多重免疫测试平台,利用这些新型光进行高通量诊断.
- 为了研究光谱和潜在分辨率ECL信号的多重化能力.
主要方法:
- 新的Ru (II) 和Ir (III) 复合物的合成和表征.
- 调查单个和混合光体ECL行为 (强度,光谱,图像).
- 开发一种无斑点的多重免疫试验,使用带有光荷载的聚合物珠和均的三明治免疫反应.
- 使用潜在和频谱双分辨率ECL进行信号读取.
主要成果:
- 成功合成了几种新的Ru (II) 和Ir (III) 复合物,具有独特的ECL特性.
- 在特定的Ru和Ir复合体之间实现了高达145nm的光谱峰值分离.
- 证明了混合光的潜在解决的ECL信号.
- 在300μL样本体积中,成功地同时检测了三种抗原 (CEA,AFP,β-HCG),使用无点多重免疫试验.
结论:
- 开发的光体和多重化策略克服了当前ECL免疫测定中的局限性.
- 无点,双分辨率的ECL免疫试验为高通量生物标志物检测提供了灵敏和高效的平台.
- 这项工作有助于进一步了解多光ECL及其在减少样本消耗的临床诊断中的应用.
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