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在新皮层区域共享和独特的转录细胞类型
Bosiljka Tasic1, Zizhen Yao2, Lucas T Graybuck2
1Allen Institute for Brain Science, Seattle, WA, USA. bosiljkat@alleninstitute.org.
Nature
|November 2, 2018
概括
研究人员使用单细胞RNA测序在小鼠新皮质中发现了133种细胞类型. 大多数GABA神经元是共享的,但谷氨酸神经元是区域特异的,显示出不同的细胞类型分布.
科学领域:
- 神经科学
- 基因组学
- 细胞生物学
背景情况:
- 新皮质表现出多种细胞类型,分为不同层次和功能区域.
- 了解细胞类型的多样性对于解读大脑功能至关重要.
研究的目的:
- 在小鼠新皮层的功能上不同的区域中研究细胞类型的转录组多样性.
- 根据基因表达和远程预测建立皮质细胞类型的分类.
主要方法:
- 从主要视觉皮层和前侧运动皮层的23,822个细胞的深层单细胞RNA测序.
- 将转录组数据与逆行标记集成,以确定质神经元的投影特异性.
主要成果:
- 鉴定了133种不同的转录细胞类型.
- 几乎所有含GABA的神经元类型在两个区域之间共享.
- 大多数谷氨酸性神经元类型都特定于视觉或运动皮质.
结论:
- 在功能上不同的区域之间,皮质细胞类型的组成有显著的变化,特别是对于质神经元.
- 一个结合的转录和投影分类学提供了老鼠皮层细胞类型的全面分类.
- 这项研究提升了我们对新皮层组织和细胞类型异质性的理解.
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