绘制未经探索的链球菌生物合成地图揭示了独特的循环动机
Leah B Bushin1, Kenzie A Clark1, István Pelczer1
1Department of Chemistry , Princeton University , Princeton , New Jersey 08544 , United States.
Journal of the American Chemical Society
|November 7, 2018
概括
研究人员发现一种新的方法,细菌使用激进的S-adenosylmethionine (RaS) 酶产生复杂的. 这一发现扩大了我们对微生物生物合成能力和潜在新疗法的了解.
科学领域:
- 生物化学
- 微生物学
- 有机生物化学
背景情况:
- 类天然产品作为信号和抗生素起着至关重要的作用.
- 的不同寻常的结构修改提供了大自然生物合成和治疗潜力的洞察力.
- 在哺乳动物微生物组中发现的链球菌具有尚未探索的生物合成能力.
研究的目的:
- 调查链球菌的生物合成潜力.
- 识别新型天然产品和链球菌中的生物合成途径.
- 描述前所未有的改酶反应.
主要方法:
- 开发了一个新的生物信息搜索策略.
- 与自然产品发现相关的激素S-腺甲 (RaS) 酶超级家族.
- 在搜索策略中使用了定数感应控制.
- 专注于一个特定的生物合成位点进行详细分析.
主要成果:
- 鉴定了许多未知链球菌中的生物合成位点.
- 发现了前所未有的转化后变异.
- 标志着一个四[5,6]二醇循环模式.
- 通过单个RaS酶证明了区域和立体特异性C-C键的形成.
结论:
- 扩大了微生物在合成复杂核糖体中的已知反应.
- 突出了链球菌作为新生物活性化合物的来源的潜力.
- 进步了对激素S-腺甲酶催化反应的理解.
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