在细胞和斑马鱼中的金属离子探针的光学控制使用高度选择性的DNAzymes与上转化纳米粒子结合
Zhenglin Yang, Kang Yong Loh, Yueh-Te Chu
1Institute of Chemical Biology and Nanomedicine, College of Chemistry and Chemical Engineering , Hunan University , Changsha , Hunan 410082 , China.
Journal of the American Chemical Society
|November 15, 2018
概括
研究人员开发了一种新的离子 (Zn2+) 纳米探针,用于实时跟踪生物. 这种先进的工具允许精确的时间空间监测斑马鱼胚胎和幼虫中的离子,增强生物理解.
科学领域:
- 生物医学工程 生物医学工程
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 了解生物系统中的金属离子分布至关重要.
- 现有的方法缺乏在体内研究所需的时空分辨率.
- 离子 (Zn2+) 在许多生物过程中起着至关重要的作用.
研究的目的:
- 开发一种Zn2+特定的纳米探针,用于实时的实体内金属离子的时空跟踪.
- 克服当前用于体内金属离子检测的方法的局限性.
- 研究Zn2+在生物系统中的动态分布.
主要方法:
- 摄影中的DNA酶结合到化的升级转化纳米粒子 (UCNPs).
- 使用近红外 (NIR) 光 (980 nm) 进行深层组织透和升级到紫外线 (365 nm).
- 使用Zn2+选择性DNA酶进行基质裂解,释放光信号 (FAM).
主要成果:
- 在活细胞和斑马鱼胚胎中使用NIR激发证明了成功的Zn2+传感.
- 实现了用于跟踪Zn2+分布的高时空分辨率.
- 纳米探测器在Zn2+检测时显示了增强的光信号.
结论:
- 开发的DNAzyme-UCNP探针为体内Zn2+成像提供了一个强大的平台.
- 提供了关于Zn2+在细胞内和体内模型中的动态分布和作用的新见解.
- 能够实时监测生物成像窗口中的金属离子.
相关概念视频
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