Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gene Therapy00:59

Gene Therapy

27.6K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
What is Gene Expression?01:42

What is Gene Expression?

196.7K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.7K
Gene Families01:57

Gene Families

9.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.9K
Organization of Genes02:07

Organization of Genes

73.5K
Overview
73.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Preventing Ventilator-Associated Pneumonia: A Multicenter Cross-Sectional Study of Knowledge, Attitudes, and Practices of Medical Staff in Chinese Hospitals.

Medical science monitor : international medical journal of experimental and clinical research·2026
Same author

Diabetes-Related Metabolic Osteoarthritis: Advanced Glycation-Collagen Axis, Cartilage Stiffening, and Biomaterials-Based Therapeutic Strategies.

International journal of nanomedicine·2026
Same author

Comparative effectiveness of exercise interventions for patellar tendinopathy: a systematic review and network meta-analysis of randomized controlled trials.

BMC sports science, medicine & rehabilitation·2026
Same author

Media analysis reveals the conservation risk of lost and active fishing gear in freshwater ecosystems of Hungary.

Scientific reports·2026
Same author

Screening and validation of Lacticaseibacillus paracasei F50 as a potential biocontrol agent to inhibit Aspergillus flavus in maize: New insights into biocontrol efficacy and mechanisms.

International journal of food microbiology·2026
Same author

Evaluation of microbiological cultures in patients with acute cholecystitis.

Frontiers in surgery·2026

相关实验视频

Updated: Feb 2, 2026

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
08:32

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Published on: August 9, 2022

4.4K

将细胞分裂基因与自杀基因联系起来,以定义和改善细胞治疗的安全性

Qin Liang1,2, Claudio Monetti1, Maria V Shutova1

  • 1Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.

Nature
|November 16, 2018
PubMed
概括

这项研究引入了一种新的"安全细胞系统",将自杀基因与细胞分裂基因联系起来,从而提高细胞治疗的安全性. 这种系统量化定义了安全水平, 加快了再生医学的临床转化.

更多相关视频

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
11:16

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Published on: February 15, 2019

8.1K

相关实验视频

Last Updated: Feb 2, 2026

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
08:32

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Published on: August 9, 2022

4.4K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
11:16

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Published on: February 15, 2019

8.1K

科学领域:

  • 生物医学工程
  • 复原医学
  • 遗传学

背景情况:

  • 人类多能细胞系具有治疗潜力,但需要严格的安全措施来临床使用.
  • 消灭有害细胞的现有自杀基因策略缺乏安全性的定量定义.
  • 确保基于细胞的疗法的安全性对于临床应用至关重要.

研究的目的:

  • 开发一种基因组工程自杀系统,
  • 使用数学模型量化评估细胞移植疗法的安全水平.
  • 加速基于细胞的再生医学的临床转化.

主要方法:

  • 基因组工程是为了在单纯疹病毒胺激酶 (HSV-TK) 自杀基因和细胞分裂基因CDK1之间创建转录链接.
  • 基于细胞数量和基因组编辑类型量化细胞治疗安全性的数学模型的开发.
  • 在分裂细胞中保护自杀系统免于失活.

主要成果:

  • 通过将HSV-TK与CDK1连接,成功创建了一个"安全细胞系统",确保细胞分裂中的自杀基因功能.
  • 开发了一个数学框架来定量定义细胞治疗的安全水平.
  • 建议的系统在确保移植细胞的安全方面取得了重大进展.

结论:

  • 开发的"安全细胞系统"提供了一种强大的方法来提高基于人类多能细胞的疗法的安全性.
  • 通过数学建模进行定量安全评估对于临床转化至关重要.
  • 这种方法有望加速基于细胞的再生医学的临床应用.