鉴定了一种由HIV-1的vpu基因编码的蛋白质
E A Cohen1, E F Terwilliger, J G Sodroski
1Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Nature
|August 11, 1988
概括
研究人员发现了人类免疫缺陷病毒1 (HIV-1) 编码的第九种蛋白质. 这种新型HIV-1蛋白的抗体存在于感染个体中,使其与相关病毒区别开来.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 人类免疫缺陷病毒1 (HIV-1) 导致艾滋病,并表现出复杂的感染模式 (临床,潜伏,非细胞病变).
- 艾滋病毒-1基因组是复杂的,编码结构蛋白,酶和调节蛋白 (tat,art,sor,3'orf,R).
- 以前对HIV-1基因组序列的分析表明,可能存在未被发现的蛋白质.
研究的目的:
- 研究HIV-1基因组编码的以前未被发现的蛋白质的可能性.
- 为了确定这种潜在的新型蛋白质的抗体是否存在于HIV-1感染个体中.
- 评估这个蛋白质是否使HIV-1与HIV-2以及猿类免疫缺陷病毒 (SIV) 有区别.
主要方法:
- 从多个HIV-1分离体中分析核酸序列.
- 在感染HIV-1的人类血清中检测抗体.
- 与人类和猿类免疫缺陷病毒 (HIV-2和SIV) 的比较分析.
主要成果:
- 发现了HIV-1编码的第九种蛋白质的证据.
- 在HIV-1感染个体的血清中检测到对这种新型蛋白质的抗体.
- 这种第九种蛋白质是HIV-1的独特特征,因为HIV-2和SIV缺乏编码类似蛋白质的能力.
结论:
- 艾滋病毒-1编码了以前未被识别的第九种蛋白质.
- 对这种蛋白质的抗体的存在可以作为HIV-1感染的潜在生物标志物.
- 这一发现突显了HIV-1和其他相关的隐形病毒之间的遗传区别.
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