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一种新的核酸切除修复,用于将A/G不匹配转换为E. coli中的C/G基对

A L Lu¹, D Y Chang

¹Department of Biological Chemistry, University of Maryland, School of Medicine, Baltimore 21201.

|September 9, 1988

概括

研究人员在大肠杆菌中发现了一种新的DNA修复途径,可以纠正A/G不匹配. 这一途径涉及一种特定的内核酶和一种不匹配的结合蛋白,导致A转化为C.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
DNA 修复机制的修复机制

背景情况:

在复制过程中可能会出现DNA不匹配,如果不修复,会导致突变.
存在特定的途径来纠正各种DNA基对错误,但不断发现新的途径.

研究的目的:

确定和描述参与纠正大肠杆菌中A/GDNA不匹配的蛋白质.
为了阐明一个新发现的DNA修复途径独立于大和mutHLS系统的机制.

主要方法:

凝电泳DNA结合试验被用来检测与A/G不匹配结合的蛋白质.
采用染色学技术来净化和分离A/G不匹配结合蛋白和相关的内核酶.
进行了DNA裂变测试,以确定内核酶的特异性和切口部位.

主要成果:

在大肠杆菌中发现了一种特别结合A/G不匹配的蛋白质.
净化了与不匹配结合蛋白相关的特定内核酶.
内核酶在A / G不匹配中切割DNA,在特定的基结对着腺素,促进单向A转化为C.

结论:

一个新的,独立于dam和mutHLS的DNA修复途径纠正了大肠杆菌中的A/G不匹配.
标志性内核酶和不匹配结合蛋白是该途径的关键组成部分.
提出了一个核酸切除修复模型,用于将A/G不匹配转换为C/G基对.

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