分离了编码S. cerevisiae热冲击转录因子的基因
G Wiederrecht1, D Seto, C S Parker
1Division of Chemistry, California Institute of Technology, Pasadena 91125.
Cell
|September 9, 1988
概括
研究人员分离了酵母热冲击转录因子基因 (HSF1). 这种必不可少的单拷贝基因编码了一种具有缺乏已知的结构动机的DNA结合域的蛋白质,这对于细胞应激反应至关重要.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 转录因子研究 转录因子研究
背景情况:
- 热冲击转录因子 (HSF) 是细胞应激反应的关键调节者.
- 了解HSF1的结构和功能是解读压力下的基因调节的关键.
研究的目的:
- 为了隔离和表征酵母热冲击转录因子基因 (HSF1).
- 为了确定HSF1蛋白的DNA结合域.
主要方法:
- 使用lambda gt11.11进行基因组图书馆选.
- DNA 测序和蛋白质质量测定.
- 在大肠杆菌和酵母菌中的功能表达.
- 南方分析和基因破坏实验.
主要成果:
- 成功分离和测序了HSF1基因,编码了833个氨基酸蛋白质.
- 表达的蛋白质显示了特定的DNA结合和转录活性,证实了基因的同一性.
- HSF1是酵母的一副本,是酵母中必不可少的基因.
- 将DNA结合域映射到118个氨基酸区域.
结论:
- 酵母HSF1基因已经被克隆和表征.
- HSF1的DNA结合域不符合已知的DNA结合结构图案.
- HSF1是酵母活力的重要基因.
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