与内质网关联的无膜器官使3'UTR介导的蛋白与蛋白相互作用成为可能
1Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell
|November 20, 2018
概括
其他3'未翻译区域 (3'UTR) 允许蛋白质的多功能性. 研究人员发现TIS颗粒和内网形成TIGER域,增强蛋白质相互作用和多样性.
科学领域:
- 分子生物学
- 细胞生物学
- 生物化学
背景情况:
- 在人类基因中存在替代3'未翻译区域 (3'UTR),通过3'UTR介导的相互作用实现蛋白质的多功能性.
- 了解3'UTR信息如何影响蛋白质功能对于破译细胞复杂性至关重要.
研究的目的:
- 研究3'UTR向蛋白质传递信息的机制.
- 识别参与调节蛋白质-蛋白质相互作用和功能的新型细胞区.
主要方法:
- 对RNA结合蛋白TIS11B及其相关结构的表征.
- 显微镜和生物化学测试以分析TIS颗粒形成及其与内质网膜 (ER) 的关联.
- 研究TIGER域在调解蛋白质与蛋白质相互作用和蛋白质表面表达中的作用.
主要成果:
- 结合RNA的蛋白质TIS11B形成了TIS颗粒,它们是含有富含AU元素的mRNA丰富的无膜细胞器.
- TIS颗粒与ER结合,形成一个名为TIGER域的独特亚细胞区.
- TIGER域促进了SET和膜蛋白之间的3'UTR介导相互作用,增强了CD47和PD-L1等蛋白质的表面表达和功能多样性.
结论:
- TIGER域代表了一个新的亚细胞区,促进了特定且功能相关的蛋白质-蛋白质相互作用.
- 通过调解3'UTR驱动的相互作用,该区增强了蛋白质表面表达和功能多样性.
- 这些发现揭示了影响蛋白质功能和细胞过程的新层后转录调节.
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