相关实验视频
Updated: Feb 2, 2026

06:06
In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
6.0K
在strigolactone信号中D3-D14泛素联酶的结构可塑性
Nitzan Shabek1,2,3, Fabrizio Ticchiarelli4, Haibin Mao1,2
1Department of Pharmacology, University of Washington, Seattle, WA, USA.
Nature
|November 23, 2018
概括
植物激素叫做strigolactones调节植物的生长. 由strigolactones控制的D14酶和D3蛋白相互作用导致D53抑制剂的降解,抑制了芽的分支.
科学领域:
- 植物激素信号
- 植物分子生理学
- 蛋白质无处不在
背景情况:
- 黄素是调节各种生理过程的关键植物激素.
- D14 酸酶会代谢干细胞,并与 F 盒蛋白 D3 相互作用.
- 这种相互作用针对D53转录抑制剂进行全方位化和降解,抑制树枝的分支.
研究的目的:
- 阐明由D14和D3介导的荷尔蒙依赖的D53无化机制.
- 了解D14如何与D3在strigolactone信号中发挥作用.
- 揭示D14,D3和D53之间相互作用的结构基础.
主要方法:
- 对D3蛋白的结构分析.
- 生物化学测试以研究蛋白质与蛋白质的相互作用.
- 研究酶活性调节.
- 分析依赖于斯特里戈拉克的无处不在性.
主要成果:
- D3蛋白具有具有两个不同的形状状态的C端α螺旋.
- 这种"结"状态有助于D3,D14和水解的strigolactone中间体的结合.
- 这种"脱离"状态将未经修改的D14结合起来,抑制其酶活性,并使得依赖于strigolactone的D53的招募成为可能.
- 这揭示了SCFD3-D14的结构可塑性.
结论:
- D3 C端α螺旋体的形状灵活性是协调strigolactone信号和新陈代谢的关键.
- 一种机制被提出,在这种机制中,E3连接酶的活性是通过strigolactone结合和蛋白质相互作用来调节的.
- 这项研究提供了关键植物激素信号通路的结构性见解.
相关概念视频
Plasticity
3.1K
Plasticity is the property where an object loses its elasticity and undergoes irreversible deformation, even after the deformation forces are eliminated. If a material deforms irreversibly without increasing stress or load, then this is called ideal plasticity. For example, when a force is applied to an aluminum rod, it changes its shape, but it does not return to its original shape once the force is removed. Plastic deformation or ductility is thus a permanent deformation or change in the...
3.1K
Plasticizers
370
Water-reducers, or plasticizers, are chemical admixtures used in concrete to improve strength and workability. These additives reduce the water-cement ratio without compromising workability, lower the cement content while maintaining the same workability, or increase workability to assist concrete placement in inaccessible areas.
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
370
Plastic Behavior
577
A material's elastic behavior is characterized by the disappearance of stress once the load is removed, allowing the material to return to its original state. However, when stress surpasses the yield point, yielding commences, marking the onset of plastic deformation or permanent set. This change from elastic to plastic behavior is influenced by the peak stress value and the duration before the load is removed. An intriguing observation occurs when a specimen is loaded, unloaded, and...
577
Plastic Deformations
444
It is essential to understand how structural members behave under plastic deformation when the bending stress exceeds the material's yield strength. This state of deformation permanently alters the shape of the member, in contrast to the linear elastic behavior observed before yielding. The strain at any point in the member is expressed in terms of maximum strain. Notably, the neutral axis, which coincides with the centroid during elastic bending, shifts away from the centroid under plastic...
444
Plastic Deformations
466
Plastic deformation represents a fundamental concept in materials science, which explains the irreversible change in the shape of a material when it experiences stress beyond its elastic capability. This phenomenon is important in structural engineering, especially in designing and analyzing cantilever beams—structures that are securely fixed at one end and bear loads at the opposite end. When these beams are subjected to loads within their elastic range, they will return to their...
466
What is Cell Signaling?
130.4K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
130.4K

