生物工程心血管绕道移植的快速自组装从支架稳定,管状胆叶细胞板
Daniel von Bornstädt1, Hanjay Wang1, Michael J Paulsen1
1Departments of Cardiothoracic Surgery (D.v.B., H.W., M.J.P., A.B.G., A.E., A.T., L.S., A.N.S., V.N.T., K.J., C.H., Y.J.W.), Stanford University, CA.
Circulation
|November 27, 2018
概括
这项研究引入了一种新技术,用于加速使用可生物降解的组织脚架生产自组装的工程血管管道. 这种方法将生产时间显著缩短到两周,从而使心血管旁路移植的临床应用成为可能.
科学领域:
- 生物材料科学
- 血管生物学
- 组织工程
背景情况:
- 自主动脉和静脉导管用于心血管旁路移植因患者可用性而受到限制.
- 自组合的单细胞移植提供现成的可用性,但生产时间较长,阻碍了临床使用.
- 加快工程血管管道的产生对于临床转换至关重要.
研究的目的:
- 引入一种新的技术,可显著加速自组装工程血管导管的生产.
- 评估这些快速生成的导管在心血管旁路移植中的有效性和安全性.
主要方法:
- 人类大动脉光滑肌细胞和纤维细胞的胆层细胞被包裹在针头周围,形成管状结构.
- 一种经过临床认可的生物降解组织膜作为一种临时的支架,用于生物反应器中的即时 perfusion 和 endothelialization.
- 在大鼠大腿动脉插入移植模型中评估了工程血管管道,评估了突破压力,血管活性,流动力学,透气性和组织学.
主要成果:
- 与不稳定的对照相比,粘膜稳定管道的成熟时间是五分之一.
- 在植入后8周,管道表现出生理血管活性,破裂压力超过500mmHg,通透率为100%.
- 组织学分析显示了成熟的动脉结构,包括明显的层和弹性膜.
结论:
- 这种新的技术将人工血管导管生产时间缩短到两周, 与心血管手术的临床时间表一致.
- 该方法使用经过临床批准的材料,可适应各种细胞来源,促进未来的临床转化.
- 迅速生成的基于细胞的血管移植显示出治疗动脉样硬化的潜力.
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