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相关概念视频

C4 Pathway and CAM01:27

C4 Pathway and CAM

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Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
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Other Glycolytic Pathways01:24

Other Glycolytic Pathways

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The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
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Auditory Pathway01:15

Auditory Pathway

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Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...
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ER Retrieval Pathway01:45

ER Retrieval Pathway

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In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
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Respiration Pathways01:26

Respiration Pathways

780
Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
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Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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相关实验视频

Updated: Feb 2, 2026

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

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在细胞染色体P450中绘制基质识别路径

Navjeet Ahalawat1, Jagannath Mondal1

  • 1Tata Institute of Fundamental Research, Center for Interdisciplinary Sciences , Hyderabad 500107 , India.

Journal of the American Chemical Society
|November 28, 2018
PubMed
概括

细胞染色体P450酶使用单一的主导途径进入基质,涉及基质诱导的结构变化而不是大型蛋白质开口. 这说明了基质如何到达这些重要的金属酶的催化中心.

科学领域:

  • 生物化学
  • 酵素学
  • 结构生物学

背景情况:

  • 细胞染色体P450是药物代谢和解毒的关键金属酶.
  • 基质进入其活性部位的机制尚不清楚,特别是考虑到封闭的晶体结构.

研究的目的:

  • 调查P450的形状异质性是否导致基质获取途径变化.
  • 阐明基质进入P450催化中心的机制.

主要方法:

  • 原子分子动力学模拟 (多微秒).
  • 对自发基质与P450cam相关性的分析.
  • 马尔科夫状态建模和自由能量分析.

主要成果:

  • 在P450cam中,基质的识别通过单一,明确的主导途径发生.
  • 基板结合包括一个关键表面通道和基板诱导的侧链位移.
  • 触媒中心的访问不需要大规模的蛋白质开放.

结论:

  • 细胞染色体P450基质的识别是由基质诱导的结构重组驱动的.
  • 建立了一个全面的基质访问模型,使以前的发现相协调.

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