用Neurite-SELEX生成的抗L1CAM Aptamer对神经细胞网络进行成像
Linlin Wang1,2, Tao Bing1,2, Ying Liu1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190 , China.
Journal of the American Chemical Society
|November 29, 2018
概括
研究人员开发了一种DNA吸收体, yly12, 针对神经细胞及其相关的L1CAM蛋白. 这种aptamer有助于可视化神经网络并抑制神经细胞的增长,为神经科学研究提供了一个新的工具.
科学领域:
- 神经科学
- 生物技术
- 分子生物学
背景情况:
- 神经细胞外生是神经系统发育的关键因素,也是评估神经毒性和神经再生的关键因素.
- 开发针对神经细胞的特定分子探针对于这些评估至关重要.
研究的目的:
- 使用修改后的SELEX策略开发用于神经细胞的DNA体探针.
- 识别aptamer的分子标并评估其在神经科学研究中的有用性.
主要方法:
- 使用神经元特异性的SELEX (指数式丰富对联体的系统演化) 策略来生成DNA体.
- 选择了aptamer yly12,并确定了它的结合性标.
- 在活细胞成像,脑组织切片成像和神经元增长抑制试验中测试了aptamer的有效性.
主要成果:
- 一个DNA的aptamer,yly12,被产生强烈地与神经细胞结合.
- yly12的分子标被确定为神经细胞粘附分子L1 (L1CAM).
- yly12成功地对活细胞和脑组织中的神经元网络进行了成像,并证明了神经元外生长的抑制作用.
结论:
- yly12 DNA 体是神经科学研究的强大分子工具,特别是用于可视化神经细胞和研究它们的发育.
- 针对亚细胞结构的神经元- SELEX 策略是有效的.
- yly12有可能用于神经退行性疾病研究和神经再生药物的开发.
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