酵母U2 snRNA的两个保存域由945个非必不可少的核酸分开
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|October 7, 1988
概括
酵母U2小核RNA (snRNA) 有不同的功能域. 删除酵母U2 snRNA的中部区域对生长没有影响,而改变3'端会影响细胞活力.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 酵母U2小核RNA (snRNA) 比其哺乳动物对应物大得多.
- 了解酵母U2 snRNA的功能域对于阐明它在细胞过程中的作用至关重要.
研究的目的:
- 调查大酵母U2 snRNA分子内不同域的功能意义.
- 确定酵母U2 snRNA对于细胞活力和功能所需的重要区域.
主要方法:
- 删除分析被用来系统地去除酵母U2 snRNA的部分.
- 现型分析,包括增长率评估,对具有删除U2 snRNA区域的酵母菌株进行.
- 对U2 snRNA 3'端的分析是在3'终端域中缺失的细胞中进行的.
主要成果:
- 酵母U2 snRNA分子可以在功能上被分为三个不同的域.
- 删除高度保守的5'域 (大约. 120核酸) 是致命的,证实了它的重要作用.
- 令人惊的是,删除大型中央域 (945个核酸) 不会影响酵母菌的生长速度.
- 删除3'终端域 (110个核酸) 中的序列导致增长缓慢和U2 snRNA 3'末端发生变化,这表明它在稳定性方面发挥了作用.
结论:
- 酵母U2 snRNA至少具有两个功能关键元素:基本的5'域和参与粒子形成的3'域.
- 酵母U2 snRNA的5'域直接参与了拼接反应.
- 酵母U2 snRNA的3'域可能在产生和稳定小核核核糖核蛋白颗粒 (snRNP) 中发挥关键作用.
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Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
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Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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