在活细胞中绘制本地和全球液相行为,使用可光聚合的种子
Dan Bracha1, Mackenzie T Walls1, Ming-Tzo Wei1
1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Cell
|December 1, 2018
概括
细胞可以使用液-液相分离来创建局部结构,即使蛋白质量很低. 这可以通过在核化部位集中蛋白质来实现,从而绕过细胞内组织的全球度限制.
科学领域:
- 生物物理
- 细胞生物学
- 分子生物学
背景情况:
- 液相分离 (LLPS) 对于形成细胞内结构至关重要.
- 对于LLPS的精确定位,特别是对于低丰度蛋白质,尚不清楚.
- 控制局部LLPS的生物物理原理尚不清楚.
研究的目的:
- 研究局部细胞内分离的生物物理机制.
- 绘制细胞内相图并了解蛋白质序列依赖的过渡机制.
- 探索低丰度蛋白质如何驱动局部的LLPS.
主要方法:
- 开发一种叫做"Corelets"的仿生寡合化系统.
- 使用光控制的可调性进行细胞内相图的快速和定量映射.
- 将实验方法与计算模拟相结合.
主要成果:
- 细胞内度可能不足以实现全相分离.
- 将蛋白质连接物绑定到核化中心可以诱导局部相分离.
- 一个扩散捕获机制使LLPS独立于全球蛋白质丰度.
结论:
- 在没有高总蛋白度的情况下可以实现局部分离.
- 扩散捕获机制是细胞内凝聚物的关键.
- 这种机制为细胞提供了一种方法来克服凝结物形成的蛋白质丰度的限制.
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