氧化降解酶活动在血-非血双核工程机械化肌球蛋白中通过一电子降解循环
Sinan Sabuncu1, Julian H Reed2, Yi Lu2
1Department of Biochemistry & Molecular Biology , Oregon Health & Science University , Portland , Oregon 97239 , United States.
Journal of the American Chemical Society
|December 5, 2018
概括
工程化肌球蛋白模型 (FeBMbs) 模仿细菌氧化减少酶 (NOR). 这些模型显示了NOR与各种金属离子的活性,支持氧化减少的单电子通路.
科学领域:
- 生物化学 生化学
- 生物有机化学 生物有机化学
- 酶模仿的酶模仿者
背景情况:
- 细菌的氧化减少酶 (NOR) 对循环至关重要.
- 肌球蛋白工程已经产生了FeBMbs,这是NORs的功能模型.
- 这些模型具有模仿NORs的二铁活性部位.
研究的目的:
- 研究工程FeBMb1蛋白中的NOR循环.
- 探索不同金属离子在FeB位点中的作用.
- 为减少氧化的反应途径提供证据.
主要方法:
- 构建含有FeBMb1蛋白质的单甲基血.
- 装载FeB网站使用FeII,CoII,或ZnII.
- 里埃变换红外光谱 (FTIR) 检测N2O的产品.
主要成果:
- 观察到多个氧化还原酶的回转.
- FeII-FeBMb1(MF-heme) 显示了最大的营业额.
- 在FeB位点与ZnII保持NOR活动.
- FTIR提供了N2O.的定量测量.
结论:
- FeBMbs是研究NOR机制的可行的功能模型.
- 结果支持一电子半减小路径用于NO减小.
- 双核中心在减少条件下有效减少氧化.
相关概念视频
Oxidation-Reduction Reactions
75.7K
Oxidation–Reduction Reactions
75.7K
Nitric Oxide Signaling Pathway
6.3K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.3K
Oxidation and Reduction of Organic Molecules
9.4K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
The removal of an electron from a molecule, results in a...
9.4K
Reactions at the Benzylic Position: Oxidation and Reduction
5.0K
The benzylic position describes the position of a carbon atom attached directly to a benzene ring. Benzene by itself does not undergo oxidation. In contrast, the benzylic carbon is quite reactive in the presence of strong oxidizing agents such as KMnO4 or H2CrO4. Therefore, alkylbenzenes are readily oxidized to benzoic acid, irrespective of the type of alkyl groups.
5.0K
Pyruvate Oxidation
168.8K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.8K
Electron Transport Chains
112.2K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
112.2K


