开发基于活动的十一转位家族酶探针
Journal of the American Chemical Society
|December 7, 2018
概括
新的化学工具利用十一转位 (TET) 酶基质的杂乱性. 这些工具可以直接追踪DNA中的TET酶活性,帮助表观遗传调节研究.
科学领域:
- 生物化学
- 分子生物学
- 表观遗传学
背景情况:
- 十-十一转位 (TET) 酶对于表观遗传调节至关重要,在DNA中催化5-甲基细胞酸氧化.
- 目前用于探测TET活性的化学工具是有限的,因为在复杂的DNA基质中追踪反应性是具有挑战性的.
研究的目的:
- 开发用于直接追踪十一转位 (TET) 酶活性的新型化学工具.
- 为了开发新的检测方法,利用TET酶基质的杂乱性.
主要方法:
- 对非自然5位修饰的细胞因子,特别是5-维尼尔细胞因子 (vC) 和5-乙烯基细胞因子 (eyC) 的TET酶活性进行了研究.
- 开发了用于标记终点读取的vC氧化中的5-甲基甲基产品的方法.
- 利用eYC氧化中的基中间体形成以共价捕获活性TET异型.
主要成果:
- TET酶随意氧化VC形成5-甲基细胞素,使得标记和活性读取有效.
- 与eyC的TET酶反应产生一种选择性地捕获活跃的TET异型的ketene中间体.
- 这种捕获方法即使在复杂的细胞溶解物中也是有效的,允许直接可视化TET活动.
结论:
- 利用TET酶基质的杂乱性提供了直接追踪TET活动的新和必要的手段.
- 这些化学工具为研究体外和体内TET功能提供了新的方法.
- 开发的方法提高了研究TET酶在表观遗传调节中的作用的能力.
更多相关视频
06:51High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
13.7K
08:17A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
Published on: April 16, 2021
2.8K
相关概念视频
Protein Families
16.9K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key...
16.9K
Protein Families
4.5K
4.5K
Enzymes
94.7K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.7K
Gene Families
9.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.9K
Gene Families
3.8K
3.8K
Enzymes and Activation Energy
23.6K
The activation energy (or free energy of activation), abbreviated as Ea, is the small amount of energy input necessary for all chemical reactions to occur. During chemical reactions, certain chemical bonds break, and new ones form. For example, when a glucose molecule breaks down, bonds between the molecule's carbon atoms break. Since these are energy-storing bonds, they release energy when broken. However, the molecule must be somewhat contorted to get into a state that allows the bonds to...
23.6K
