细胞透传输非联热激活延迟光纳米颗粒用于时间分辨率发光成像
Zece Zhu, Di Tian, Pengli Gao1
1Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM) , Nanjing University of Posts and Telecommunications , Nanjing , 210023 , China.
Journal of the American Chemical Society
|December 12, 2018
概括
研究人员开发了细胞透纳米粒子用于先进的生物成像. 这些生物相容的纳米粒子能有效地穿越细胞膜,即使在富含氧气的环境中也能清晰地进行成像.
科学领域:
- 纳米技术
- 生物医学成像
- 材料科学
背景情况:
- 具有较长激发状态寿命的发光探针和纳米粒子对于时间解析的生物成像至关重要.
- 细胞膜对许多非自然化合物的吸收构成重大障碍,挑战了有效的成像剂的开发.
- 在生物相容,寿命长的发光纳米粒子,特别是非金属纳米粒子中实现高细胞膜透性仍然是一个关键挑战.
研究的目的:
- 设计和合成具有增强细胞膜透性的新型生物相容纳米粒子,用于定时发光成像.
- 通过细胞透,研究疏水性光体的自我组装到稳定的纳米粒子中.
- 评估这些纳米粒子在生物成像应用中的有效性,特别是在有氧环境中.
主要方法:
- 用于输送疏水性光体的两性细胞透F6G6R3R2的设计.
- 三个热激活延迟光 (TADF) 分子 (4CzIPN,NAI-DPAC,BTZ-DMAC) 与一起组装成水溶液中分散的纳米粒子.
- 对纳米粒子细胞毒性和膜透性的评估.
- 在氧气环境中进行时间分辨率发光成像的演示.
主要成果:
- 设计的可以从恐水性TADF分子中形成稳定,分散的纳米颗粒.
- 由此产生的纳米颗粒具有较低的细胞毒性和高效的细胞膜透.
- 从TADF纳米颗粒的长寿命发光使得在氧气的存在下成功的时间分辨率成像.
- 与共价方法相比,非共价相互作用在纳米粒子传递方面是有效的,提供了灵活性和方便性.
结论:
- 该研究使用TADF分子和细胞透成功开发了生物相容的细胞透纳米粒子.
- 这些纳米粒子有望在生物系统中实现先进的时间分辨率发光成像,克服膜障碍.
- 这些发现突显了细胞透的非共价传递分子和纳米颗粒的潜力,扩大了它们的应用范围.
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